BI Spectroscopy as a Research Tool
3.5.2.
This technique is a powerful research tool for study of:
- Quality and properties of chromatographic supports
- Optimization of enzyme capture on solid supports
- Optimizing conditions for fluorescence tagging
- Improving protocols for bioligand immobilization on solid support, such as
stationary phase or biosensor sensing
- Characterization of nonselective binding on solid phase supports
Features of BI as a research tool are highlighted in this section by case study #1, which discovered the flaws in the widely used protocol for protein immobilization.
And by case study #2, investigating binding capacity of Sepharose 4B beads with immobilized Streptavidin as the ligand.
